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skfE [2019-06-06 12:32:20]
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skfE [2019-06-06 12:32:20]

[SW|ABC transporter] (binding protein), export of the spore killing factor
locus
BSU01950
pI
6.34
mw
26.75 kDa
protein length
239 aa Sequence Blast
gene length
720 bp Sequence Blast
function
export of the spore killing factor [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|SkfA]
product
[SW|ABC transporter] (binding protein)
essential
no
ec
3.6.3.25
synonyms
ybdA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    216,913 217,632

    Phenotypes of a mutant

  • inactivation of ''[gene|5886364199844356446C08F08664B301A714E74A|skfE]'' reduces sporulation efficiency to 54% that of wild type cells [Pubmed|26735940]

    • The protein

    Protein family

  • [SW|ABC transporter superfamily] (according to UniProt)

    • Structure

  • [PDB|4RVC] (from Geobacillus kaustophilus, 33% identity) [pubmed|25724946]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH]
    description
    [Pubmed|16816204]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16452424], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|14651647,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|15687200,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|16816204], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

    • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]

    • additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|SkfA]'-specific transcript, and a weaker 6.5 kb [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH] transcript
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C515 (ybdA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2513 NBRP B. subtilis, Japan]
  • BKE01950 ([gene|5886364199844356446C08F08664B301A714E74A|skfE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA, downstream forward: _UP4_TAATATCATTTCTTTTTTAT
  • BKK01950 ([gene|5886364199844356446C08F08664B301A714E74A|skfE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA, downstream forward: _UP4_TAATATCATTTCTTTTTTAT

    • References

    Reviews

  • 20955377

    • Original Publications

  • 11731129,10092453,12817086,16816204,14651647,15687200,15687200,17720793,17449691,18840696,26735940,25724946